Title : Analysis of biopharmaceutical properties of cuminum cyminum, Syzgym aromaticum and Capsicum annuum against Bovine mastitogens
The Present study is aimed at evaluating in vitro activity of ethnolic, n-hexane andacetone extracts of medicinal plants; Cumminum cyminum, Syzgium aromaticum and Capsicum annuum against bovine mastitogens. Bacterial mastitogens isolated from milk samples and their strains were confirmed by morphological studies were Escherichia coli, S.aeureus and Kleibsella. Extracts of selected plant materials (C.cyminum seed; S.aromaticum flower buds and C.annum fruits) were prepared by maceration technique. Formulations of different extracts were prepared to evaluate their synergistic or antagonistic effects. Extract and formulations were tested against mastitogens using agar disc diffusion method and zones of inhibition were measured to compare efficacy of these plants. It was observed that combined plant extracts showed enhanced antibacterial activity. Most of the combined plants extract formulations revealed synergism. Our findings reported that clinical strains E. coli, S. aureus and Kleibsella which were completely resistant to ciprofloxacin showed tremendous susceptibility towards separate and combined plant extracts. Most susceptible strain towards plant extracts were Kleibsella E. coli followed by S. aureus. Most resistant strain towards plant extracts was Kleibsella. Ethanol shows maximum efficacy individually but in formulation it reduces its efficacy. Capsicum annuum shows no efficacy in individual trial while in combination C.annuum showed maximum efficacy (C.annuumHex 5μl+C.annuumAce 5μl+ C.annuumEth 5μl) Hex 1:1:1. Topmost three largest zones of S.aromaticum was observed against S.aureus including extracts S.aromaticumHex 5μl, S.aromaticumAce 5μl, S.aromaticumEth 5μl (1:1:1) gave the highest zone of 40mm. It was concluded that prominent synergism occurred in most of the combined plant extracts highlighting medical importance for combating infections caused by multidrug resistant bacteria. Current study clearly suggests that various combinations of these three plant extract could be used as potential natural resources in order to develop promising antibiotic for treating various infections.
Audience take away:
- They can test extracts of the selected plant varieties against resistance Bovine mastitogens, reported in their area.
- Pharmacologist can develop new drug against from the extracts of selected plants.
- In Vivo analysis can be done further to evaluate any toxic effects of these plants.