Title : Modified Antibiotic susceptibility testing (abst) for evaluating bacterial resistance in biofilm forming bacterial isolates from chronics mastitis
Abstract:
Bacterial isolates from 3600 milk samples chronic mastitis of cattle were screened over a period of march 2019 to December 2022 were subjected to routine isolation and identification. Antibiotic sensitivity testing of all samples for both biofilm faoming and planktonic forms were also carried out and It was revealed that styphylococcus (n=3156) E. Coli (n=3552) and P. aeruginosa (n=457) were the predomint isolation. The result showed that bacterial isolates from prolonged chronic mastitis were isolated and screened for their biofilm producing ability. Antibiotic sensitivity testing for both biofilm forming and planktonic forms of selected isolates of Staphylococcus aureus (n=7), E. coli (n=5) and P. aeruginosa (n=4) was performed. 0.3% Bentonite clay was adopted as a substrate along with MHA for biofilm forming bacterial isolates. The results showed that average zone of inhibition and resistance against antibiotics was higher for biofilm forming bacterial isolates than their planktonic counterparts. Therapeutic resistance is much higher for bacteria that is commonly observed in nutrient agar media during screening for antibiotic sensitivity testing, suggesting there is a need for use of other substrates. Keywords; Antibiotics resistance; bentonite clay; biofilm; canine; wound
