Title : Sero-prevalence of contagious bovine pleuro-pneumonia disease of dairy cows at Barishal region, Bangladesh
Respiratory diseases of cattle are very costly and deadly disease and among them contagious bovine pleura-pneumonia (CBPP) is a silent killer, that was listed an OIE, caused by Mycoplasma mycoides subsp. mycoides, a member of the Mycoplasma mycoides. The aim of the present study was to evaluate the seroprevalence of CBPP antibody and analysis the risk factors associated with the CBPP in dairy cattle. Total eleven dairy farms in 4 upazilas of Barisal division were selected. From this dairy farm total 91 blood samples were collected and serums were preserved at -20C for further use. The ELISA kit for CBPP antibody was used to detect CBPP in the collected sample according to manufacture instructions and 450 nm filter was used to read the ELISA microplate. The results were interpreted according to the supplied literature. DNA was extracted by Favorgen blood genomic DNA extraction mini kit for polymerase chain reaction (PCR). Among the samples about 39.56% samples were positive for CBPP antibody in this area. However, among the 4 upazilas, 52.38% samples were positive for CBPP antibody at Mehendiganj upazila and lowest CBPP antibody was detected at Barishal sadar upazila. According to cow level data analysis, it was found that numerically Holstein cattle were more susceptible to CBPP than Sahiwal cattle and female animals were more susceptible than male animals but there was no any significant difference. On the other hand, good farm hygiene practice can reduce CBPP antibody detection at dairy farm level. However, 21.98% sample were positive for CBPP in PCR result. In this study it was also found that CBPP antibody was more detected in small scale dairy farms in regards to either herd size or farm area. From this study it was concluded that CBPP antibody was more detected in Holstein cattle and good farm hygiene practice and large farm area can reduced the chance of CBPP infection.
Acknowledgement: This work was supported by a grant from the Bangladesh Bureau of Educational Information and Statistics, Ministry of Education, Bangladesh.