HYBRID EVENT: You can participate in person at London, UK or Virtually from your home or work.

International Conference on
Veterinary Science

August 28-30, 2023 | London, UK
VET 2023

Hazem Abdo Abubaker

Hazem Abdo Abubaker, Speaker at veterinary congress
University of Khartoum, Sudan
Title : Evidence of multiple point mutations in Theileria annulata cytochrome b gene incriminated in buparvaquone treatment failure

Abstract:

Drug resistance is one of the emerging and re-emerging epidemics affecting both veterinary and public health sectors. Buparvaquone provides the most satisfactory means in the treatment of bovine tropical theileriosis. However, recently there has been widespread reports of development of resistance of Theileria annulata to buparvaquone. To investigate the situation in Sudan where bovine tropical theileriosis is endemic, fifty blood samples from T. annulata-positive cattle. were used for DNA extraction, PCR and cytochrome b gene nucleotide sequencing. Analysis of the two buparvaquone binding site regions Q01 (130–148) and Q02 (244–266), revealed three non- synonymous mutations at codon 146; alanine (GCT) to threonine (ACT) within the Q01 region across all 50 isolates and the other mutation at codon 129; serine (AGC) to glycine (GGC) in 18 isolates which is very close to the Q01 binding site. However, we documented another mutation at position 227; valine (GTG) to methionine (ATG) close to the close to the Q02 binding site, in three isolates with mutation at codon 129. We concluded that this study has provided evidence of point mutations in the cytochrome b gene of T. annulata that might be associated with buparvaquone treatment failure in Sudan.

  1. Emergence of resistance to buparvaquone by some identified theileria annulata parasite in Sudan.
  2. The failure of buparvaquone treatment might be due to mutations in cytochrome b gene.
  3. Evidence of mutations in Theileria annulata were reported in other countries.
  4. Advanced molecular techniques would help considerably to identify mutations that cause failure of treatment.
  • Explain how the audience will be able to use what they learn?

They can apply the molecular biology techniques in further research studies.

  • How will this help the audience in their job?

To consider failure of treatments that can happen frequently in veterinary therapeutics when they are treating animals.

  • Is this research that other faculty could use to expand their research or teaching?

Yes, by conducting further research on causes of drug resistance.

  • Does this provide a practical solution to a problem that could simplify or make a designer’s job more efficient?

Confirming emergence of resistance to buparvaquone would help to draw attention to develop new drug for Theileriosis.
Will it improve the accuracy of a design, or provide new information to assist in a design problem?

Using of molecular biology techniques was very efficient to determine the problem behind buparvaquone resistance.

Other benefits could be gained from this research are screenings of other parasites to identify mutations could be relevant to emergence of drug resistance.

Biography:

Hazem Abdo Abubaker studied at the University of Khartoum, Sudan and graduated as Veterinarian in 2016. I joined to the department of clinical medicine, faculty of veterinary medicine, university of Khartoum to work as a teaching assistant in 2017. I got my master degree in 2020 by completion of research in veterinary science (medicine). I have been participated in several research articles ranging from molecular virology and parasitology to bioinformatics.

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