HYBRID EVENT: You can participate in person at London, UK or Virtually from your home or work.

International Conference on
Veterinary Science

August 28-30, 2023 | London, UK
VET 2023

Shalini Sharma

Shalini Sharma, Speaker at Veterinary Conference 
GADVASU, India
Title : Studies on the gene expression profile of the immune regulatory genes in peripheral blood mononuclear cells of Cattle naturally infected with bovine tuberculosis.

Abstract:

Bovine tuberculosis (bTB) is a chronic infectious disease caused primarily by infection with M. bovis, which belongs to the Mycobacterium tuberculosis complex. The initial host response against bTB infections is mainly regulated by the Th1 response, which is characterized by the production of IFN-γ. With the progression of disease, Mycobacterium can survive in the host through the evasion of the host’s immune response by manipulating it. Whole blood based assays, particularly interferon gamma (IFN-γ) release assays (IGRAs), are used for the diagnosis of bTB. The aim of the current study was to evaluate a panel of immuneregulatory genes for their potential use as the diagnostic biomarkers of M. bovis infection in cattle. In the present study a total number of 168 cattle were screened by comparative intradermal tuberculin test and molecular confirmation of  bTB was done using IS6100 PCR. The haematological analysis of  (n=25) bTB positive and (n=25) bTB negative cattle was performed.  PBMCs from (n=25) naturally infected cattle and (n=25) healthy cattle was cultured and stimulated with Bovine PPD used as the stimulating antigen in vitro. PBMCs were harvested after 16hrs stimulation with the stimulating antigen. Seven target genes (MCP-1, MMP-9, CCR5, iNOS, IFNγ, TNFα and IL-23A) and one reference gene (GAPDH) was studied by the SYBR green RT-PCR assay. Cattle positive for bTB has reduced total lymphocytes count along with increased TLC and granulocytes. All the target genes were significantly upregulated (p<0.05) in bTB positive cattle as compared to the healthy cattle. Findings suggest that (MCP-1, MMP-9, CCR5, iNOS, IFNγ, TNFα and IL-23A) can be consider as the potential diagnostic biomarkers of bTB infection in cattle.

Biography:

Dr. Shalini Sharma studied BVsc & A.H and graduated  in 2011 from CSKHPKV Palampur, Himachal Pradesh India. MVsc in Veterinary microbiology and immunology was done in same institute in 2013. She was appointed as Veterinary Officer  from year 2014-2018 in Himachal Pradesh state government. PhD degree was received  in 2022 at GADVASU Ludhiana, India. ICAR NET qualified in 2018. She got AIR-4th in SRF-PGS Examination for PhD entrance in 2018. Global Eminent Researcher Award 2021 from Vij Education Trust was received.  Award of INSPIRE Fellowship from DST in 2014 for completion of PhD. She has four published original research articles one review article as first author from MVsc research. She reviewed more than 15 research articles. She has 5 conference proceedings papers and two book chapters now she is working on her PhD research articles.

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